PhD, University of Konstanz, Germany (1994);
Postdoctoral Fellow, Oregon State University (1994 - 99);
Assistant Professor, Utrecht University, The Netherlands (1999 - 02)
Mass Spectrometry Facility Website:
We focus on mass spectrometry to study biomolecules, their interactions and dynamics in relation to their biological functions. We develop analytical strategies to study the response of biological systems to exposure, stress and disease. Specifically, we explore the metabolome and proteome as comprehensive collections of molecular messengers of cellular response. For this purpose we combine chemistry, separation technologies and mass spectrometry to detect, identify and quantify biomolecules in biological systems.
Current research includes
1. Gas phase and fragment ion chemistry, structure elucidation;
2. Analytical method development and quantitative mass spectrometry for applications in the environmental, plant and biomedical sciences;
3. Analytical metabolomics with focus on metabolite profiling using ion mobility mass spectrometry for biomarker discovery;
4. Proteomics techniques for studying oxidative and electrophile stress, redox signaling and homeostasis, chronic diseases and aging;
5. Mass spectrometry-enabled protein conformational and interaction analysis with emphasis on H/D exchange mass spectrometry.
- Tandem mass spectrometric characterization of thiol peptides modified by the chemoselective cationic sulfhydryl reagent (4-iodobutyl)triphenylphosphonium--effects of a cationic thiol derivatization on peptide fragmentation. J. Am. Soc. Mass Spectrom. 2011, 22(10):1771-83
- Site-specific proteomic analysis of lipoxidation adducts in cardiac mitochondria reveals chemical diversity of 2-alkenal adduction. J. Proteomics. 2011, 74(11):2417-29.
- Conformational studies of the robust 2-Cys peroxiredoxin Salmonella typhimurium AhpC by solution phase hydrogen/deuterium (H/D) exchange monitored by electrospray ionization mass spectrometry. Nirudodhi S, Parsonage D, Karplus PA, Poole LB, Maier CS. Int. J. Mass Spectrom. 2011, 302(1-3):93-100.
- Mass spectrometry-based quantification of myocardial protein adducts with acrolein in an in vivo model of oxidative stress. Mol. Nutr. Food Res. 2011 55(9):1401-10
- A targeted mass spectrometry-based approach for the identification and characterization of proteins containing α-aminoadipic and γ-glutamic semialdehyde residues. Anal. Bioanal. Chem. 2010, 398(7-8):2905-14.
- Protein adducts of aldehydic lipid peroxidation products identification and characterization of protein adducts using an aldehyde/keto-reactive probe in combination with mass spectrometry. Maier CS, Chavez J, Wang J, Wu J. Methods Enzymol. 2010; 473:305-30.