M. S. (2005) Texas A&M University - Commerce;
Ph.D. (2011) University of Texas - Austin;
Postdoctoral Associate (2011 - 2013) University of Michigan - Ann Arbor
Robert A Welch Fellowship Grant from Texas A&M University - Commerce
Lochte Fellowship Grant from Department of Chemistry in the University of Texas at Austin
Travel award from 4th ASM Conference on Cell-Cell Communication in Bacteria, Miami, FL
Research Group Website
Our group is highly interdisciplinary in the nature of our research efforts. Our main focus is to develop novel electrochemical techniques to investigate new frontiers in microbiology, such as the social behavior of microbes, otherwise known as quorum sensing. This collective behavior of bacteria, or quorum sensing, plays an important role in forming biofilm, which ultimately helps bacteria collectively overcome many adverse environmental conditions, including making the microbes resistant to antibiotics. We are interested in using new electrochemical techniques such as scanning electrochemical microscopy (SECM) to study the microbial metabolites in real-time and in 3D space to elucidate the microbial behavior in the context of biofilm related diseases (cystic fibrosis) and infections in/on biomedical devices (catheters, artificial heart valves etc.). These findings will ultimately lead us to design the next generation smart biomedical devices.
We are also actively involved in exploring the possibilities of applying varied electrochemical techniques to study the complex electron transfer processes in microbial fuel cell.
Ummadi, J. G.; Joshi, V. S.; Gupta, P. R.; Indra, A. K.; Koley, D. Single-Cell Migration as Studied by Scanning Electrochemical Microscopy. Anal. Methods 2015. DOI: 10.1039/C5AY01944C
Ren, H.; Colletta, A.; Koley, D.; Wu, J.; Xi, C.; Major, T. C.; Bartlett, R. H.; Meyerhoff, M. E. Thromboresistant/anti-Biofilm Catheters via Electrochemically Modulated Nitric Oxide Release. Bioelectrochemistry 2014, 104C, 10–16. DOI: 10.1016/j.bioelechem.2014.12.003
Höfler, L.; Koley, D.; Wu, J.; Xi, C.; Meyerhoff, M. E. Electromodulated Release of Nitric Oxide Through Polymer Material from Reservoir of Inorganic Nitrite Salt. RSC Adv. 2012, 2, 6765–6767. DOI: 10.1039/C2RA20853A
Koley, D.; Bard, A. J. Inhibition of the MRP1-Mediated Transport of the Menadione-Glutathione Conjugate (thiodione) in HeLa Cells as Studied by SECM. Proc. Natl. Acad. Sci. U. S. A. 2012, 109, 11522–11527. DOI: 10.1073/pnas.1201555109
Koley, D.; Ramsey, M. M.; Bard, A. J.; Whiteley, M. Discovery of a Biofilm Electrocline Using Real-Time 3D Metabolite Analysis. Proc. Natl. Acad. Sci. U. S. A. 2011, 108, 19996–20001. DOI: 10.1073/pnas.1117298108
Liu, X.; Ramsey, M. M.; Chen, X.; Koley, D.; Whiteley, M.; Bard, A. J. Real-Time Mapping of a Hydrogen Peroxide Concentration Profile across a Polymicrobial Bacterial Biofilm Using Scanning Electrochemical Microscopy. Proc. Natl. Acad. Sci. U. S. A. 2011, 108, 2668–2673. DOI: 10.1073/pnas.1018391108
Koley, D.; Bard, A. J. Triton X-100 Concentration Effects on Membrane Permeability of a Single HeLa Cell by Scanning Electrochemical Microscopy (SECM). Proc. Natl. Acad. Sci. U. S. A. 2010, 3–5. DOI: 10.1073/pnas.1011614107